From Plant Press, Vol. 26, No. 4, October 2023.
-Contributions from Gary Krupnick, Laurence Dorr, Erika Gardner, Rose Gulledge, Alice Tangerini, Jun Wen, Kenneth Wurdack, and Robert Costello.
While the COVID-19 pandemic resulted in quiet hallways and empty offices, the return to the office has led to in-person activity. The summer months are a conundrum—some staff travel to international conferences and field expeditions, but new voices fill the herbarium as a new crop of interns and volunteers make their presence known. The summer of 2023 was no exception, with a handful of eager individuals learning and contributing to science in the Department of Botany. Here we present an overview of the fresh faces that visited this summer for training and research.
The Natural History Research Experiences (NHRE) is a 10-week summer research internship program for undergraduates at the National Museum of Natural History funded by the National Science Foundation. From May 30 to August 4, Botany hosted two NHRE interns, Jillian Cieslik and Luke Sparreo. NHRE interns work closely with scientific mentors on independent research projects. Project results were presented at a poster session for NMNH staff, as well as during a public outreach day in the exhibit halls, and the results are being prepared for publication.
Larry Dorr, Jillian Cieslik, and Kenneth Wurdack with Lindera specimens in the herbarium. (photo by Smithsonian Institution)
Kenneth Wurdack and Larry Dorr mentored Jillian Cieslik, a senior at Ball State University (Indiana) with interests in plant microbiology and genetics. Her project, “Establishing phylogenetic relationships and distinctions of the North American Lindera,” was to sort out a complex of native spice bushes using molecular techniques. She examined relationships among North American spicebushes (three species of Lindera and two species of Litsea; Lauraceae), with a special emphasis on species delimitation in the bog spicebush, Lindera subcoriacea, a rare southeastern USA wetland species, which has been considered questionably distinct from the widespread Lindera benzoin. Plastid genomes were assembled, and several nuclear genes were sequenced that resolved relationships of these species and confirmed that L. subcoriacea should be treated as a distinct species. Cieslik plans to continue her focus on Botany and attend graduate school next year.
Natural History Research Experiences (NHRE) intern Luke Sparreo of Connecticut College collecting Vitis simpsonii (Sparreo #1) on Plummers Island. (photo by Jun Wen).
Jun Wen, Greg Stull, and Alicia Talavera hosted NHRE intern Luke Sparreo from Connecticut College. Sparreo worked on the project “Discovering a new genus of the grape family Vitaceae and exploring tropical-temperate transition in plants.” This project focused on a potential new plant genus, Neoampelocissus, and its relationship with other genera in the grape plant family, Vitaceae. The phylogenomic results from the study show that the Neoampelocissus group is sister to the grape genus Vitis rather than the genus Ampelocissus, where the small group of c. six species of Neotropical Ampelocissus species have been placed. The Neoampelocissus group is found in tropical regions of Central America, while most species of its likely sister group, Vitis, occur in the north temperate regions. DNAs of Neoampelocissus species from silica gel preserved samples and herbarium specimens were sequenced using the target enrichment approach, targeting 1013 nuclear genes. The newly generated sequence data were compared with previously analyzed species of the grape family to place the new genus into a greater phylogeny. Seeds of Neoampelocissus and its close allies were imaged microscopically and measured for 14 diagnostic characteristics to add a morphological component to the project. The results support a New World origin for the Neoampelocissus – Vitis group, involving a tropical-temperate transition for the temperate radiations of the grape genus Vitis. Sparreo will apply for graduate school in Botany soon.